AIE-Gelgreen Nucleic Acid Stain
AIE-Gelgreen Nucleic Acid Stain
Product Introduction
AIE-Gelgreen Nucleic Acid Stain (10,000X) is a triphenylamine derivative based on Aggregation-Induced Emission (AIE) fluorescent materials, possessing typical AIE characteristics. It is a nucleic acid gel stain with high sensitivity, strong stability, broad applicability, and excellent compatibility, suitable for staining dsDNA, ssDNA, and RNA in agarose and polyacrylamide gels. AIE-Gelgreen exhibits extremely high sensitivity, with a detection limit as low as 1 ng, making it an ideal alternative to silver staining. It can produce clear and bright bands within 5 minutes, with simple operation and intuitive results. Its broad excitation spectrum is compatible with various imaging systems, allowing it to replace ethidium bromide (EB) and other gel stains without altering existing equipment. It is suitable for gel imaging and detection systems originally using nucleic acid stains such as SYBR Green or SYBR Gold. Moreover, AIE-Gelgreen can be used for nucleic acid detection with harmless blue light lamps or blue light imaging devices, avoiding the mutagenic effects of conventional UV detection on nucleic acid samples as well as the harm to the eyes and skin from UV exposure, making it an ideal choice for use with laser scanners.
Storage Conditions
Please store at 2-8°C, light protection is not required. The shelf life is 1 year.
Product Properties
Formula C33H30N4O22+
Molecular Weight 514.63 g/mol
Purity >95% (HPLC)
Working Concentration 5-10 μM
Maximum Absorption/Emission Wavelength (nm) Ex=435 nm / Em= 580 nm
Application Scope
It is used for staining double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and RNA in agarose and polyacrylamide gel electrophoresis.
Product Features
1. High Sensitivity: Suitable for nucleic acid electrophoresis staining of fragments of various sizes, with sensitivity up to 1 ng;
2. Strong Stability: Can be heated in a microwave, and its effectiveness remains unaffected after more than one year of storage at room temperature;
3. Broad Compatibility: Matches standard gel imaging systems and visible light excitation gel observation devices;
4. Fast and Simple: Clear and bright bands can be obtained within 5 minutes of staining, without the need for destaining or rinsing;
5. Strong Operability: Maintains a high signal-to-noise ratio within a concentration range of 0.1X to 1X;
6. Broad Applicability: Suitable for agarose or polyacrylamide gel electrophoresis, capable of staining dsDNA, ssDNA, or RNA.
Manufacturer: GDSBio