DMEM/F12 Medium
DMEM/F12 Medium
with L-Alanyl-L-Glutamine, with phenol red, with sodium bicarbonate, without HEPES, sterile-filtered, suitable for cell culture
Catalog number: LF-KR-ACE01107
Product Form: Clear solution at 1x
Product size: 500 mL
Sterility: Sterile-filtered
pH: 7.20~7.80
Endotoxin (LAL test): ≤ 5 EU/mL
Shipment: Ambient
Storage: Store at 2-8 °C for 12 months
Application: DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.
Usage instruction: DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For scientific research use only, not for clinical diagnosis or treatment.