DMEM High Glucose Medium
DMEM High Glucose Medium
high glucose (4.5 g/L), with L-Alanyl-L-Glutamine, with phenol red, with sodium bicarbonate, w/o HEPES, sterile-filtered, suitable for cell culture
Catalog number: LF-KR-ACE01103
Product Form: Clear solution at 1x
Product size: 500 mL
Sterility: Sterile-filtered
pH: 7.20~7.80
Endotoxin (LAL test): ≤ 5 EU/mL
Shipment: Ambient
Storage: Store at 2-8 °C for 12 months
Application: Dulbecco's Modified Eagle Medium (DMEM) is widely used in cell culture as a basal medium. It provides basic nutrients for growth of various mammalian cell lines, especially when supplemented with FBS. DMEM was shown to successfully cultivate primary cells, such as fibroblasts, neurons, HUVECs, smooth muscle cells, or even cells lines, such as HEK-293, HeLa, Cos-7, and PC-12.
Usage instruction: DMEM comes in various formulations, where the composition of components may differ. The original formulation of DMEM contains 1.0 g/L. DMEM High Glucose has an increased glucose content of 4.5 g/L. DMEM High Glucose contains no proteins, lipids, or growth factors. Therefore, DMEM High Glucose may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM High Glucose uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For scientific research use only, not for clinical diagnosis or treatment.