Fast Multiplex Probe qPCR Mix
Fast Multiplex Probe qPCR Mix
Product Introduction
Fast Multiplex Probe qPCR Mix is a 2X Master Mix designed for multiplex real-time quantitative PCR using probe-based detection. The reaction can be performed simply by adding template, primers, and probes. This product contains an antibody-modified Fast Hotstart Taq DNA polymerase and leverages GDSBio's specialized PCR buffer to facilitate fast multiplex ultra-high-sensitivity qPCR. The reagent incorporates a dUTP/UDG contamination prevention system, which can eliminate dUTP-containing PCR products before amplification, effectively avoiding quantitative interference caused by cross-contamination of amplification products. This product is convenient for developing fully pre-mixed Master Mix molecular diagnostic kits with probes and primers. It is compatible with various fluorescent probes such as TaqMan and works seamlessly with common quantitative PCR instruments from manufacturers like ABI, Roche, and Bio-Rad. This product allows reaction setup without an ice box—the PCR Mix, primers, and template can be prepared at room temperature, and the prepared PCR reaction system can be stored at room temperature for 24 hours without loss of amplification efficiency.
Applications
• Probe gene expression analysis
• Probe Low-copy gene detection
• Fast Multiplex probe qPCR
• IVD diagnostic kit research
Features
• Ready-to-use 2X Master Mix
• Fast, Multiplex & Highly Sensitive
• Built-in Contamination Control
• Broad Compatibility
• Room-Temperature Stable
Components
2X Fast Multiplex Probe qPCR Mix: 1 ml
Storage Condition
This reagent can be stored for 2 months at 4°C. For longer storage, it should be kept at -20°C. Avoid repeated freezing and thawing.
The Fast SYBR Green Blue qPCR Mix (Universal ROX+), with Fast Hotstart Taq Polymerase as its core component, can complete qPCR reactions within 45 minutes.
Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucl- eases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in amplification of a single-copy gene from human genomic DNA.
Product Use Limitations
This product is sold exclusively for research purposes and in vitro use. Neither the product, nor any individual components, was tested for use in diagnostic applications or for drug development, nor is it suitable for administration to humans or animals. Please refer to the MSDS, available upon request.
Manufacturer: GDSBio