Fast SYBR Green Blue qPCR Mix (Universal ROX+)
Fast SYBR Green Blue qPCR Mix (Universal ROX+)
Product Introduction
Fast SYBR® Green Blue qPCR Mix (Universal ROX+) is a dye-based qPCR Master Mix pre-mixed with blue loading indicator and universal ROX. It eliminates the need for instrument-specific ROX concentration adjustments, simplifying operation. Utilizing a mutant hot-start Taq DNA polymerase, it enables highly sensitive rapid amplification while minimizing interference from primer dimers and by-products, significantly enhancing the specificity, amplification efficiency, and quantifiable dynamic range of quantitative PCR. Incorporated with a novel enhancer, it minimizes variability in PCR efficiency across different target amplicons and exhibits negligible impact on amplification performance after repeated freeze-thaw cycles. This product is fully compatible with common quantitative PCR instruments, such as those from ABI, Roche, and Bio-Rad.
The reaction system of this product can be prepared at room temperature without an ice box. The prepared PCR reaction system can be placed at room temperature for 24 hours and the amplification efficiency remains unchanged.
Applications
• Gene expression analysis
• Low-copy gene detection
• Microarray validation
• Gene knockdown validation
Features
• Fast and stable amplification performance
• Contains universal ROX reference dye
• Compatible with many Real-time systems
• Hot-start technology brings high specificity and reproducible amplification
Components
2X Fast SYBR® Green Blue qPCR Mix (Universal ROX+) *: 1 ml
Nuclease-free Water: 1 ml
* contains SYBR® Green I, Fast Hotstart Taq DNA polymerase, universal ROX reference dye, dNTP, reaction buffer, etc.
Storage Condition
This reagent can be stored for 2 months at 4°C and protected from light. For longer
storage, it should be kept at -20°C and protected from light. Avoid repeated freezing and thawing.
Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucl- eases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in amplification of a single-copy gene from human genomic DNA.
Product Use Limitations
This product is sold exclusively for research purposes and in vitro use. Neither the product, nor any individual components, was tested for use in diagnostic applications or for drug development, nor is it suitable for administration to humans or animals. Please refer to the MSDS, available upon request.
Manufacturer: GDSBio