Recombinant Human BDNF Protein (animal-free)

Alternate Names

Brain-derived Neurotrophic Factor; Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin.

Characteristics

Species

Human

Expression System

Chinese Hamster Ovary (CHO) cell line

Fusion Tag

Tag Free

Protein Accession

P23560, Human BDNF (His129-Arg247)

Predicted Molecular Mass

13.5 kDa

Specification

Appearance

White powder, colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin Level

≤10 EU/mg by the LAL method

Biological Activity

Determined by the dose-dependent proliferation of C6 cells and is typically between 0.5-1.5 ug/mL, corresponding to a specific activity of 1 x 10^3 units/mg.

Formulation

Lyophilized from a 0.22 um filtered solution containing PBS,5% mannitol and 0.01% Tween 80, pH7.4

Size

10 μg/vial, 50 μg/vial, 1 mg/vial

Handling and Storage

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.