Recombinant Human BMP-2 Protein (animal-free, DMF-041476)

Alternate Names

Bone Morphogenetic Protein 2; BDA2; BMP2; BMP-2; BMP-2A; BMP2A; Bone morphogenetic protein 2A; SSFSC.

Characteristics

Species

Human

Expression System

E. coli

Fusion Tag

Tag Free

Protein Accession

P12643, Human BMP-2 (Gln283-Arg396)

Predicted Molecular Mass

12.9 kDa (monomer)

Specification

Appearance

White powder, colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin Level

≤100 EU/mg by the LAL method

Biological Activity

Measured alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 is ≤0.5 μg/ml.

Formulation

Lyophilized from a 0.2 μm filtered solution containing 0.1M Acetic acid, 5% Trehalose, pH 3.0

Size

10 μg/vial, 50 μg/vial, 1 mg/vial

Handling and Storage

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.