Recombinant Human BMP-7 Protein (animal-free)

Alternate Names

Bone morphogenetic protein 7; BMP-7; BMP7; Osteogenic protein 1 (OP-1); BMP7; OP1; Eptotermin α; Eptotermin alfa.

Characteristics

Species

Human

Expression System

Chinese Hamster Ovary (CHO) cell line

Fusion Tag

Tag Free

Protein Accession

P18075, Human BMP-7 (Met316-His431)

Predicted Molecular Mass

13.1 kDa (monomer)

Specification

Appearance

White powder, colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin Level

≤10 EU/mg by the LAL method

Biological Activity

Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.02-0.04 μg/ml.

Formulation

Lyophilized from a 0.22 μm-filtered solution containing 0.085% TFA, 30% ACN, 5% mannitol

Size

10 μg/vial, 50 μg/vial, 1 mg/vial

Handling and Storage

Reconstitution

It is recommended to redissolve in 4mM HCl.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.