Recombinant Human Epidermal Growth Factor

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Download COA Batch 20240523008

 

Alternate Names	Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; epidermal growth factor (beta-urogastrone); hEGF; HOMG4; Urogastrone; eta-urogastrone; URG; Urogastrone.
Characteristics
Species	Human
Expression System	Yeast (Pichia Pastoris)
Fusion Tag	Tag Free
Molecular Weight	6 kDa
Specification
Appearance	White or off-white loose body
Purity	≥98%, by SDS-PAGE
Endotoxin Level	< 1.0 EU/mg
Biological Activity	≥1.0 x 10^6 IU/mg by a cell proliferation assay using Bal B/C 3T3 cell
Formulation	Purified by chromatography from E.coli, then lyophilized with Mannitol.
Size	50 µg, 1 mg, and 10 mg
Handling and Storage
Reconstitution	Recommend to reconstitute at 100-200μg/ml in sterile water. Further dilutions can be made in other aqueous buffer.
Shipping	The product is shipped with blue ice.
Storage & Stability	Stored at temperature -20°C for at least 36 months. Please avoid repeated freeze-thaw cycles.

 

Product Description

Human epidermal growth factor (EGF) is a 6 kDa protein with 53 amino acid residues and three intramolecular disulfide bonds. By binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface, EGF results in a variety of biochemical changes within the cell: a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR that ultimately lead to DNA synthesis and cell proliferation.

The rhEGF, purified by chromatography from Yeast, then lyophilized with Mannitol, has high stability and biological activity. The manufacture capability at hundreds of grams scale can meet your large bioprocessing needs.

 

Advantages

Chemically Defined & Animal-Free;
High Batch-to-Batch Consistancy;
Stable Bulk Supply
cGMP Manufactured

 

Application

Cell Culture
IVD Reagent Antigens

 

FOR RESEARCH, LABORATORY AND MANUFACTURE USE ONLY. NOT INTENDED FOR DIRECT USE ON HUMANS.

 

Manufacturer: Wuhan Healthgen (Oryzogen)