Recombinant Human Fibronectin (plant-derived)
Recombinant Human Fibronectin (plant-derived)
Alternate Names
Fibronectin, FN.
Characteristics
Species
Human
Expression System
Rice Grain (Oryza Sativa)
Fusion Tag
Tag Free
Molecular Weight
200-250 kDa
Specification
Appearance
White lyophilized powder
Purity
≥95%, by SDS-PAGE
Endotoxin Level
<0.5 EU/µg
Formulation
Lyophilized powder, purified from rice endosperm.
Size
1g and 10g
Handling and Storage
Reconstitution
Recommend to reconstitute in the sterile water or PBS with 1mg/ml concentration. Further dilutions can be made in other aqueous buffer.
Shipping
The product is shipped with blue ice.
Storage & Stability
Stored at temperature -20°C for at least 36 months. Please avoid repeated freeze-thaw cycles.
Fibronectin (FN) is a particularly important and well-studied component of the extracellular matrix, and is known to play a key role in cell adhesion, growth, spreading, migration, differentiation and proliferation. Fn is a 200-250 kDa glycoprotein composed of 2 subunits bound via a disulfide bond. Currently, the Fn is purified from the plasma, which however is limited by the availability of supply. The the recombinant human fibronectin (OsrhFN) was expressed in the rice endosperm platform, which is animal component free and has high purity, and has been demonstrated has the same physical and chemical with the plasma derived Fn. OsrhFN provides a safety solution to replace the plasma derived FN.
Applications:
OsrhFN is generally used in the concentration range of 1–5 micrograms per cm2 of growth surface for attachment or at 5-50 micrograms per ml as a media additive.
(Optimal working dilutions must be determined by end user.)
Storage and Handling:
Stored at temperatures -20℃ for at least 36 months. For the lyophilized OsrhFN, add sterile 1ml of Sodium Citrate solution (20mM Citrate, 250mM NaCl, pH 7.20) to the vial to reconstitute the Fibronectin (recommended reconstituted concentration is 1mg/ml), shake gently to dissolve the lyophilized powder. The reconstituted OsrhFN liquid can be made in other aqueous buffer. Upon reconstitution OsrhFN should be stored below -20℃. Use as soon as possible after opening. Please avoid freeze-thaw cycles.