Recombinant Human IGF-II Protein (animal-free)

Alternate Names

Insulin-like Growth Factor II; C11orf43; GRDF; IGF-2; IGF2; IGF-II; IGFII; MSA; PEG2; PP9974.

Characteristics

Species

Human

Expression System

HEK293 cells

Fusion Tag

Tag Free

Protein Accession

P01344.1, Human IGF2 (Ala25-Glu91)

Predicted Molecular Mass

7.5 kDa

Specification

Appearance

White powder, colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin Level

≤10 EU/mg by the LAL method

Biological Activity

Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is ≤50 ng/mL.

Formulation

Lyophilized from a 0.22 µm filtered solution containing TFA, ACN, 5% Trehalose

Size

10 μg/vial, 50 μg/vial, 1 mg/vial

Handling and Storage

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.