Recombinant Human IL-10 Protein (animal-free)
Recombinant Human IL-10 Protein (animal-free)
Alternate Names
Interleukin-10; interleukin 10; CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; TGIF.
Characteristics
Species
Human
Expression System
Chinese Hamster Ovary (CHO) cell line
Fusion Tag
Tag Free
Protein Accession
P22301, Human IL-10 (His25-Ser162)
Predicted Molecular Mass
18.6 kDa
Specification
Appearance
White powder, colorless clear liquid after reconstitution
Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin Level
≤10 EU/mg by the LAL method
Biological Activity
Measured in a cell proliferation assay using MC-9 mouse mast cells. The ED50 is ≤ 1 ng/ml.
Formulation
Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size
10 μg/vial, 50 μg/vial, 1 mg/vial
Handling and Storage
Reconstitution
It is recommended to redissolve in sterile deionized water.
Shipping
The product is shipped with blue ice.
Storage & Stability
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.