Recombinant Human IL-1A Protein (animal-free)

Alternate Names

Interleukin 1 alpha; BAF; IL-1A; IL-1F1; IL1; IL1 alpha; IL1A; IL1F1; LAF; LEM.

Characteristics

Species

Human

Expression System

E. coli

Fusion Tag

Tag Free

Protein Accession

P01583, Human IL1A (Ser113-Ala271)

Predicted Molecular Mass

18.0 kDa

Specification

Appearance

White powder, colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin Level

≤100 EU/mg by the LAL method

Biological Activity

Measured by its ability to induce NF-kB reporter activity by the reporter gene assay. The ED50 for this effect is ≤ 0.05ng/mL.

Formulation

Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, 5% Trehalose and 0.01% Tween 80, pH 7.4

Size

50 μg/vial, 1 mg/vial

Handling and Storage

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.