Recombinant Human M-CSF Protein (animal-free)
Recombinant Human M-CSF Protein (animal-free)
Alternate Names
Macrophage Colony Stimulating Factor; CSF-1; CSF1; Lanimostim; MCSF; M-CSF.
Characteristics
Species
Human
Expression System
E. coli
Fusion Tag
Tag Free
Protein Accession
P09603.3, Human M-CSF (Glu33-Ser190)
Predicted Molecular Mass
18.4 kDa
Specification
Appearance
White powder, colorless clear liquid after reconstitution
Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin Level
≤100 EU/mg by the LAL method
Biological Activity
The bioactivity determined by the dose-dependent proliferation of murine NFS-60 cells is ＜ 0.5 ng/mL, corresponding to a specific bioactivity of ≥ 1 x 107 units/mg.
Formulation
Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, 5% Trehalose and 0.01% Tween 80, pH7.4
Size
10 μg/vial, 50 μg/vial, 1 mg/vial
Handling and Storage
Reconstitution
It is recommended to redissolve in sterile deionized water.
Shipping
The product is shipped with blue ice.
Storage & Stability
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.