Recombinant Human TNF-alpha Protein (animal-free)

Alternate Names

Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFα; TNF-α; TNFSF2.

Characteristics

Species

Human

Expression System

Chinese Hamster Ovary (CHO) cell line

Fusion Tag

Tag Free

Protein Accession

P01375, Human TNFα (Val77-Leu233)

Predicted Molecular Mass

17.4 kDa

Specification

Appearance

White powder, colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin Level

≤10 EU/mg by the LAL method

Biological Activity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 5-60 pg/mL.

Formulation

Lyophilized from a 0.22 μm-filtered solution containing 100 mM Glycine, 150 mM NaCl, 5% mannitol and 0.01% Tween 80, pH 4.0

Size

10 μg/vial, 50 μg/vial, 1 mg/vial

Handling and Storage

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.