Recombinant Human Wnt-3a Protein (animal-free)

Alternate Names

Wnt-3a; Wnt3a; MG/VAIL C119418; MG/VAIL C119419; MG/VAIL C119420; wingless-type MMTV integration site family member 3A.

Characteristics

Species

Human

Expression System

Chinese Hamster Ovary (CHO) cell line

Fusion Tag

C-Fc

Protein Accession

NP_149122, Human Noggin (Ser19-Lys352) with a C-terminal linker GGGGGS and Human lgG1 (Asp104-Lys330)

Predicted Molecular Mass

58.2 kDa

Specification

Appearance

White powder, colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin Level

≤10 EU/mg by the LAL method

Biological Activity

Measured by its ability to induce Topflash reporter bioactivity in CHO-K1 cells. The ED50 for this effect is ＜30 ng/mL.

Formulation

Lyophilized from a 0.22 μm-filtered solution containing PBS, pH 7.4

Size

10 μg/vial, 50 μg/vial, 1 mg/vial

Handling and Storage

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.