RPMI/1640 Medium
RPMI/1640 Medium
with L-Alanyl-L-Glutamine, with phenol red, with sodium bicarbonate, without HEPES, sterile-filtered, suitable for cell culture
Catalog number: LF-KR-ACE01105
Product Form: Clear solution at 1x
Product size: 500 mL
Sterility: Sterile-filtered
pH: 7.20~7.80
Endotoxin (LAL test): ≤ 5 EU/mL
Shipment: Ambient
Storage: Store at 2-8 °C for 12 months
Application: RPMI 1640 Medium was originally developed at Roswell Park Memorial Institute to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
Usage instruction: RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For scientific research use only, not for clinical diagnosis or treatment.