Super TaqMix
Super TaqMix
Product Introduction
Super TaqMix is a universal and easy-to-use PCR Master Mix that employs antibody-modified hotstart technology, offering excellent specificity and sensitivity. This product uses a universal annealing temperature of 55°C, which reduces the optimization steps of the reaction and enables simultaneous amplification of different PCR reactions. By innovatively combining a novel buffer, high-performance Taq DNA polymerase, and an excellent hot start technology, outstanding PCR results can be achieved even under the most demanding experimental applications. The amplified products have 3'-dA overhangs, which can be directly used for TA cloning.
Product Advantages
• Universal primer annealing temperature (°C) - enables simultaneous amplification of different PCR reactions
• Fast DNA synthesis speed and inhibitor tolerance - using modified Taq DNA polymerase
• Hot start technology - provides excellent specificity, sensitivity, and yield
• Green buffer - allows direct gel loading of PCR products for electrophoresis, helping to reduce pipetting errors
Super TaqMix boasts an exceptional amplification efficiency, capable of successfully amplifying target fragments from as little as 0.1 ng of human genomic DNA (Figure 1, using 0.1/0.5/2/10/50 ng of human genomic DNA as templates to amplify a 300 bp DNA fragment). It efficiently and specifically amplifies commonly sized DNA fragments (Figure 2, amplifying DNA fragments of 600/800/1000 bp), easily meeting daily PCR requirements. Super TaqMix can perfectly amplify DNA fragments with a GC content ranging from 28% to 82% (Figure 3), not only yielding high amounts but also producing specific products with a clean background.
Components
2X Super TaqMix: 1 ml
Storage Condition
Store at -20°C, protect from light, and avoid repeated freezing and thawing.
Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucl- eases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in amplification of a single-copy gene from human genomic DNA.
Product Use Limitations
This product is sold exclusively for research purposes and in vitro use. Neither the product, nor any individual components, was tested for use in diagnostic applications or for drug development, nor is it suitable for administration to humans or animals. Please refer to the MSDS, available upon request.
Manufacturer: GDSBio